Mv. Francia et al., Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin, PLASMID, 46(2), 2001, pp. 117-127
pAD1 is a 59.3-kb plasmid in Enterococcus faecalis that has been the subjec
t of intense investigation with regard to its pheromone-inducible conjugati
on behavior as well as its contribution to virulence. Approximately two-thi
rds of the pAD1 nucleotide sequence has been previously reported. Here we r
eport on an analysis of the final similar to 22 kb, a significant portion o
f which is believed to encode structural genes associated with conjugation.
The conjugation-regulated region was also found to contain a new (second)
origin of conjugative transfer (oriT). A list of open reading frames coveri
ng the entire plasmid is presented. (C) 2001 Academic Press.