The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity

Citation
Jm. Baisden et al., The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity, ONCOGENE, 20(44), 2001, pp. 6435-6447
Citations number
76
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ONCOGENE
ISSN journal
0950-9232 → ACNP
Volume
20
Issue
44
Year of publication
2001
Pages
6435 - 6447
Database
ISI
SICI code
0950-9232(20011001)20:44<6435:TAFPAI>2.0.ZU;2-7
Abstract
The actin filament-associated protein of 110 kDa (AFAP-110) was first ident ified as an SH3/SH2 binding partner for the nonreceptor tyrosine kinase, Sr c. Subsequent data have demonstrated that AFAP-110 can interact with other Src family members. AFAP-110 contains additional protein binding modules in cluding two pleckstrin homology domains, a leucine zipper motif and a targe t sequence for serine/threonine phosphorylation. AFAP-110 interacts with ac tin filaments directly via a carboxy terminal actin-binding domain. Thus AF AP-110 may function as an adaptor protein by linking Src family members and /or other signaling proteins to actin filaments. AFAP-110 also has an intri nsic capability to alter actin filament integrity that can be revealed upon conformational changes associated with phosphorylation or mutagenesis. Rec ent data has indicated that AFAP-110 may also serve to activate cSrc in res ponse to this conformational change as well. Thus, AFAP-110 may function in several ways by (1) acting as an adaptor protein that links signaling mole cules to actin filaments, (2) serving as a platform for the construction of larger signaling complexes, (3) serving as an activator of Src family kina ses in response to cellular signals that alter its conformation and (4) dir ectly effecting actin filament organization as an actin filament cross-link ing protein. Here, we will review the structure and function of AFAP-110 as well as potential binding partners and effectors of AFAP-110's ability to alter actin filament integrity.