Objectives-To investigate the influence of personal lifestyle-such as smoki
ng and alcohol consumption-on urinary 1-hydroxypyrene (1-OHP) concentration
s in coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) a
nd to evaluate the association of 1-OHP concentrations with the genetic pol
ymorphism of several metabolic enzymes including cytochrome P-450 (CYP) 1A1
and glutathione S-tranferases (GSTs).
Methods-The study population contained 162 coke oven workers and 5S control
s employed at the largest iron and steel factory in China. Personal data we
re collected at the interview. 1-OHP in urine was measured with high perfor
mance liquid chromatography with fluorescence detection. Genetic polymorphi
sms were identified by the polymerase chain reaction (PCR) method.
Results-A positive association between excretion of urinary 1-OHP and the l
evels of exposure to PAHs was confirmed. Those people who consumed greater
than or equal to 50 g/day ethanol had significantly higher 1-OHP excretion
than did other coke oven workers (p <0.01). No significant difference in ur
inary 1-OHP was found between smokers and non-smokers, in both controls and
exposed subjects. The variant homozygotes at exon 7 of the CYP1A1 gene had
significantly higher urinary 1-OHP concentrations than other CYP1A1 genoty
pes among the exposed workers (p=0.03). There was less association between
the concentrations of 1-OHP and the GSTM1, GSTP1, or GSTT1 polymorphism.
Conclusions-The present study confirmed that urinary 1-ORP is a good biomar
ker for exposure to PAHs. Alcohol consumption affected urinary 1-OHP excret
ion. The variant genotypes of the CYP1A1 gene may result in the enhancement
of PAR metabolites. It is helpful to understand the role of individual sus
ceptibility on metabolism of carcinogens. These findings suggest that the m
odulating effect of individual lifestyle factors or genetic nature should b
e considered in future studies on occupational exposure to PAHs and in eval
uating the health risk from harmful chemicals.