Urinary 1-hydroxypyrene in coke oven workers relative to exposure, alcoholconsumption, and metabolic enzymes

Citation
J. Zhang et al., Urinary 1-hydroxypyrene in coke oven workers relative to exposure, alcoholconsumption, and metabolic enzymes, OCC ENVIR M, 58(11), 2001, pp. 716-721
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Envirnomentale Medicine & Public Health","Pharmacology & Toxicology
Journal title
OCCUPATIONAL AND ENVIRONMENTAL MEDICINE
ISSN journal
1351-0711 → ACNP
Volume
58
Issue
11
Year of publication
2001
Pages
716 - 721
Database
ISI
SICI code
1351-0711(200111)58:11<716:U1ICOW>2.0.ZU;2-A
Abstract
Objectives-To investigate the influence of personal lifestyle-such as smoki ng and alcohol consumption-on urinary 1-hydroxypyrene (1-OHP) concentration s in coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) a nd to evaluate the association of 1-OHP concentrations with the genetic pol ymorphism of several metabolic enzymes including cytochrome P-450 (CYP) 1A1 and glutathione S-tranferases (GSTs). Methods-The study population contained 162 coke oven workers and 5S control s employed at the largest iron and steel factory in China. Personal data we re collected at the interview. 1-OHP in urine was measured with high perfor mance liquid chromatography with fluorescence detection. Genetic polymorphi sms were identified by the polymerase chain reaction (PCR) method. Results-A positive association between excretion of urinary 1-OHP and the l evels of exposure to PAHs was confirmed. Those people who consumed greater than or equal to 50 g/day ethanol had significantly higher 1-OHP excretion than did other coke oven workers (p <0.01). No significant difference in ur inary 1-OHP was found between smokers and non-smokers, in both controls and exposed subjects. The variant homozygotes at exon 7 of the CYP1A1 gene had significantly higher urinary 1-OHP concentrations than other CYP1A1 genoty pes among the exposed workers (p=0.03). There was less association between the concentrations of 1-OHP and the GSTM1, GSTP1, or GSTT1 polymorphism. Conclusions-The present study confirmed that urinary 1-ORP is a good biomar ker for exposure to PAHs. Alcohol consumption affected urinary 1-OHP excret ion. The variant genotypes of the CYP1A1 gene may result in the enhancement of PAR metabolites. It is helpful to understand the role of individual sus ceptibility on metabolism of carcinogens. These findings suggest that the m odulating effect of individual lifestyle factors or genetic nature should b e considered in future studies on occupational exposure to PAHs and in eval uating the health risk from harmful chemicals.