Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1

Citation
E. Yung et al., Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1, NAT MED, 7(8), 2001, pp. 920-926
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research General Topics
Journal title
NATURE MEDICINE
ISSN journal
1078-8956 → ACNP
Volume
7
Issue
8
Year of publication
2001
Pages
920 - 926
Database
ISI
SICI code
1078-8956(200108)7:8<920:IOHVPB>2.0.ZU;2-7
Abstract
Integase Interactor 1(INI), also known as hSNF5, is a protein that interact s with HIV-1 integrase. We report here that a cytoplasmically localized fra gment of INI (S6; aa183-294) containing the minimal integrase-interaction d omain potently inhibits HIV-1 particle production and replication. Mutation s in 56 or integrase that disrupt integrase-INI1 interaction abrogated the inhibitory effect. An integrase-deficient HIV-1 transcomplemented with inte grase fused to Vpr was not affected by S6. INI1 was specifically incorporat ed into virions and was required for efficient HIV-1 particle production. T hese results indicate that INI1 is required for late events in the viral li fe cycle, and that ectopic expression of S6 inhibits HIV-1 replication in a transdominant manner via its specific interaction with integrase within th e context of Gag-Pol, providing a novel strategy to control HIV-1 replicati on.