Design and testing of a novel microscopic photon correlation spectrometer with higher accuracy

Authors
Citation
Jc. Wang, Design and testing of a novel microscopic photon correlation spectrometer with higher accuracy, J OPT A-P A, 3(5), 2001, pp. 360-365
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science","Optics & Acoustics
Journal title
JOURNAL OF OPTICS A-PURE AND APPLIED OPTICS
ISSN journal
1464-4258 → ACNP
Volume
3
Issue
5
Year of publication
2001
Pages
360 - 365
Database
ISI
SICI code
1464-4258(200109)3:5<360:DATOAN>2.0.ZU;2-R
Abstract
A novel microscopic photon correlation spectrometer has been designed and d eveloped in order to employ photon correlation spectroscopy to investigate dynamic properties within a microscopic volume. It shows a spatial resoluti on scale of about 0.5 micrometres and a relaxation timescale of millisecond s. The variance of the scattering vector is less than 5% even at a smaller scattering angle. Furthermore, a standard performance test using 94 +/- 13 nm polystyrene latex beads has been performed on the apparatus. The autocor relation function value at the first channel is higher than 0.8, that indic ates a higher signal-to-noise ratio and a good coherent detection. CONTIN a nalysis gives the latex beads size as 94 +/- 2 nm. Different angle scatteri ng results give the translation diffusion coefficient (4.57 +/- 0.08) x 10( -8) cm(2) s(-1) at 293.15 K, that agrees with the theory very well. Besides , scattering spectroscopy using different pinhole sizes determines the opti mized configuration for the spectrometer with higher accuracy. All these do cument that the designed system works much more accurately than previous sy stems and may probe dynamic information from different local sites of a sin gle living cell non-invasively.