Molecular analysis of the interaction of LCMV with its cellular receptor alpha-dystroglycan

Citation
S. Kunz et al., Molecular analysis of the interaction of LCMV with its cellular receptor alpha-dystroglycan, J CELL BIOL, 155(2), 2001, pp. 301-310
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
155
Issue
2
Year of publication
2001
Pages
301 - 310
Database
ISI
SICI code
0021-9525(20011015)155:2<301:MAOTIO>2.0.ZU;2-H
Abstract
alpha -Dystroglycan (DG) has been identified as the cellular receptor for l ymphocytic choriomeningitis virus (LCMV) and Lassa fever virus (LFV). This subunit of DG is a highly versatile cell surface molecule that provides a m olecular link between the extracellular matrix (ECM) and a beta -DG transme mbrane component, which interacts with the actin-based cytoskeleton. In add ition, DC exhibits a complex pattern of interaction with a wide variety of ECM and cellular proteins. In the present study, we characterized the bindi ng of LCMV to alpha -DG and addressed the role of alpha -DG-associated host -derived proteins in virus infection. We found that the COOH-terminal regio n of alpha -DG's first globular domain and the NH2-terminal region of the m ucin-related structures of alpha -DG together form the binding site for LCM V. The virus-alpha -DG binding unlike ECM alpha -DG interactions was not de pendent on divalent cations. Despite such differences in binding, LCMV and laminin-1 use, in part, an overlapping binding site on alpha -DG, and the a bility of an LCMV isolate to compete with laminin-1 for receptor binding is determined by its binding affinity to alpha -DG. This competition of the v irus with ECM molecules for receptor binding likely explains the recently f ound correlation between the affinity of LCMV binding to alpha -DG, tissue tropism, and pathological potential. LCMV strains and variants with high bi nding affinity to alpha -DG but not low affinity binders are able to infect CD11c(+) dendritic cells, which express alpha -DG at their surface. Infect ion followed by dysfunction of these antigen-presenting cells contributes t o immunosuppression and persistent viral infection in vivo.