Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130

Citation
Ma. Puthenveedu et Ad. Linstedt, Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130, J CELL BIOL, 155(2), 2001, pp. 227-237
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
155
Issue
2
Year of publication
2001
Pages
227 - 237
Database
ISI
SICI code
0021-9525(20011015)155:2<227:ETGSDO>2.0.ZU;2-A
Abstract
Inhibition of the putative coatomer protein I (COPI) vesicle tethering comp lex, giantin-p115-GM130, may contribute to mitotic Golgi breakdown. However , neither this, nor the role of the giantin-p115-GM130 complex in the maint enance of Golgi structure has been demonstrated in vivo. Therefore, we gene rated antibodies directed against the mapped binding sites in each protein of the complex and injected these into mammalian tissue culture cells. Surp risingly, the injected anti-p115 and antigiantin antibodies caused proteaso me-mediated degradation of the corresponding antigens. Reduction of p115 le vels below detection led to COPI-dependent Golgi fragmentation and apparent accumulation of Golgi-derived vesicles. In contrast, neither reduction of giantin below detectable levels, nor inhibition of p115 binding to GM130, h ad any detectable effect on Golgi structure or Golgi reassembly after cell division or brefeldin A washout. These observations indicate that inhibitio n of p115 can induce a mitotic-like Golgi disassembly, but its essential ro le in Golgi structure is independent of its Golgi-localized binding partner s giantin and GM130.