Modulatory effect of acetylcholine on gonadotropin-stimulated human granulosa cell steroid secretion

Citation
L. Kornya et al., Modulatory effect of acetylcholine on gonadotropin-stimulated human granulosa cell steroid secretion, GYNECOL OBS, 52(2), 2001, pp. 104-107
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
ISSN journal
0378-7346 → ACNP
Volume
52
Issue
2
Year of publication
2001
Pages
104 - 107
Database
ISI
SICI code
0378-7346(2001)52:2<104:MEOAOG>2.0.ZU;2-N
Abstract
The aim of this study was to explore the direct action of acetylcholine on gonadotropin-stimulated progesterone (P) and estradiol (E-2) secretion of h uman granulosa cells (GCs) cultured in serum-free medium. Human GCs were is olated from preovulatory follicular fluid aspirated from 22 women undergoin g in vitro fertilization at the University Women's Hospital of Tubingen. Th e production of progesterone and E-2 was measured in the presence and absen ce of acetylcholine, carbachol, atropine, luteinizing hormone (LH) or folli cle-stimulating hormone (FSH) using radioimmunoassay. Statistical analysis of the data was performed by ANOVA and Newman-Keuls test. Administration of acetylcholine or carbachol (10(-5) M) resulted in a significant increase i n P and E-2 secretion. This response was specifically blocked by the muscar inic receptor antagonist atropine. Similarly, carbachol resulted in a signi ficant increase in P and E-2 output, though the response to it was somewhat reduced when compared to that evoked by acetylcholine. Acetylcholine did n ot show any additive effect on LH-stimulated P secretion, while it augmente d the stimulatory effect of FSH on P release. In contrast, carbachol marked ly diminished the stimulatory effect of LH on P secretion, while it caused no change in FSH-induced P output. When administered together, acetylcholin e did not modify the stimulatory effect of FSH on E-2 secretion, however, i t markedly elevated LH-induced E-2 output. Similar to this, carbachol signi ficantly increased LH-induced E-2 release, however it decreased FSH-stimula ted E-2 secretion. We suggest that acetylcholine has a direct modulatory ef fect on gonadotropin-stimulated steroid production of GCs, an effect that i s mediated via muscarinic receptors. This effect may have a physiological r ole in the regulation of GC function during the menstrual cycle. Copyright (C) 2001 S. Karger AG, Basel.