Analysis of single-cell differences by use of an on-chip microculture system and optical trapping

Citation
Y. Wakamoto et al., Analysis of single-cell differences by use of an on-chip microculture system and optical trapping, FRESEN J AN, 371(2), 2001, pp. 276-281
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
ISSN journal
0937-0633 → ACNP
Volume
371
Issue
2
Year of publication
2001
Pages
276 - 281
Database
ISI
SICI code
0937-0633(200109)371:2<276:AOSDBU>2.0.ZU;2-N
Abstract
A method is described for continuous observation of isolated single cells t hat enables genetically identical cells to be compared; it uses an on-chip microculture system and optical tweezers. Photolithography is used to const ruct microchambers with 5-mum-high walls made of thick photoresist (SU-8) o n the surface of a glass slide. These microchambers are connected by a chan nel through which cells are transported, by means of optical tweezers, from a cultivation microchamber to an analysis microchamber, or from the analys is microchamber to a waste microchamber. The microchambers are covered with a semi-permeable membrane to separate them from nutrient medium circulatin g through a "cover chamber" above. Differential analysis of isolated direct descendants of single cells showed that this system could be used to compa re genetically identical cells under contamination-free conditions. It shou ld thus help in the clarification of heterogeneous phenomena, for example u nequal cell division and cell differentiation.