Polyester microchannel chip for electrophoresis - incorporation of a blue LED as light source

Citation
K. Uchiyama et al., Polyester microchannel chip for electrophoresis - incorporation of a blue LED as light source, FRESEN J AN, 371(2), 2001, pp. 209-211
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
ISSN journal
0937-0633 → ACNP
Volume
371
Issue
2
Year of publication
2001
Pages
209 - 211
Database
ISI
SICI code
0937-0633(200109)371:2<209:PMCFE->2.0.ZU;2-L
Abstract
A blue-light-emitting diode was incorporated as a fluorescence-excitation l ight source into a polyester microchannel chip fabricated by in situ polyme rization. Placing the light-emitting facet of the diode close to the microc hannel obviated any need for an additional optical arrangement. Fluorescenc e from the sample was transmitted by an optical fiber incorporated into the microchip perpendicular to the LED. FITC labeled amino acids were separate d in the presence of 5 mM SDS by using the microchip and were detected by L ED-induced fluorescence.