Is Aeromonas hydrophila the dominant motile Aeromonas species that causes disease outbreaks in aquaculture production in the Zhejiang Province of China?

Citation
Me. Nielsen et al., Is Aeromonas hydrophila the dominant motile Aeromonas species that causes disease outbreaks in aquaculture production in the Zhejiang Province of China?, DIS AQU ORG, 46(1), 2001, pp. 23-29
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aquatic Sciences
Journal title
DISEASES OF AQUATIC ORGANISMS
ISSN journal
0177-5103 → ACNP
Volume
46
Issue
1
Year of publication
2001
Pages
23 - 29
Database
ISI
SICI code
0177-5103(20010822)46:1<23:IAHTDM>2.0.ZU;2-P
Abstract
The significance of Aeromonas hydrophila in association with disease outbre aks in aquaculture production in the Zhejiang province of China was investi gated. Bacteriological examination of moribund fish and crabs resulted in 9 5 bacterial isolates: 88 bacterial isolates from fish and 7 isolates from c rabs. PCR and traditional biochemical methods were used for identification of A. hydrophila. Out of 69 motile aeromonads, 35 isolates were identified as A. hydrophila by biochemical tests. However, 6 of those were not identif ied as A. hydrophila by a species specific PCR method. Serotyping revealed 2 dominant serotypes (09 and 097) among A. hydrophila isolates. The data pr esented show that approximately 42% of the motile aeromonads isolated from disease outbreaks among various fish species were A. hydrophila. It is note worthy that A. hydrophila accounted for more than 50% of the isolated aerom onands isolated from crucian carp Carassius carassius and Wuchang bream Meg alobrama amblycephala with haemorrhagic septicaemia. Although this species was the most frequently isolated organism from internal organs of diseased fish and crabs in the present study, other motile Aeromonas spp. were also found. The PCR assay was useful in preventing misidentification of A. hydro phila, which may occur when only phenotypic tests are employed.