Isolation and partial characterization of selenium-containing tRNA from germinating barley

Citation
Kx. Huang et al., Isolation and partial characterization of selenium-containing tRNA from germinating barley, BIOL TR EL, 82(1-3), 2001, pp. 247-257
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
BIOLOGICAL TRACE ELEMENT RESEARCH
ISSN journal
0163-4984 → ACNP
Volume
82
Issue
1-3
Year of publication
2001
Pages
247 - 257
Database
ISI
SICI code
0163-4984(200122)82:1-3<247:IAPCOS>2.0.ZU;2-G
Abstract
Selenium-containing tRNA was discovered in germinating barley for the first time with the Se-75 isotopic tracer technique; therefore, this technique w as used to study the effect of different concentrations of selenium and sul fur in the medium on the content of selenium-containing tRNA in germinating barley. Se-containing tRNAs and its hydrolysates were isolated, purified, and characterized by means of column chromatography, ion-exchange chromatog raphy, high-performance liquid chromatography, and the ultraviolet-visible spectrum. The results show that the amount of selenium in tRNA is almost un affected by the sulfuric content in the medium, and the pathway for seleniu m and sulfur to enter tRNA might not be exactly the same. Selenium exists w ithin tRNA in the form of 5-methylamine methyl-2-selenouridine, just as it does within a microorganism tRNA.