Differential regulations between adenosine triphosphate (ATP)- and uridinetriphosphate-induced Cl- secretion in bovine tracheal epithelium - Direct stimulation of P1-like receptor by ATP

Citation
S. Kanoh et al., Differential regulations between adenosine triphosphate (ATP)- and uridinetriphosphate-induced Cl- secretion in bovine tracheal epithelium - Direct stimulation of P1-like receptor by ATP, AM J RESP C, 25(3), 2001, pp. 370-376
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
ISSN journal
1044-1549 → ACNP
Volume
25
Issue
3
Year of publication
2001
Pages
370 - 376
Database
ISI
SICI code
1044-1549(200109)25:3<370:DRBAT(>2.0.ZU;2-A
Abstract
Adenosine 5'-triphosphate (ATP) stimulates airway epithelial Cl- secretion in a complicated manner. We examined the difference between ATP- and uridin e 5'-triphosphate (UTP)-induced responses of short-circuit current (Isc) in bovine tracheal epithelium treated with amiloride. Each nucleotide caused an increase in Isc composed of the first and second peaks, where the second peak induced by ATP was higher compared with UTP. The ATP-induced second p eak was inhibited by the protein kinase (PK) A inhibitor H89, saturation of P1 receptor with adenosine, and the P1 receptor antagonist 8-p-sulfophenyl theophylline, but not by the Call chelator ethyleneglycol-bis-(beta -aminoe thyl ether)-N,N,N',N'-tetraacetic acid plus the endoplasmic reticulum Call- pump inhibitor thapsigargin, the adenosine breakdown enzyme adenosine deami nase, the ectonucleotidase inhibitor alpha,beta -methyleneadenosine 5'-diph osphate, or saturation of P2Y(2) receptor with UTP. Thus, the response is a ssociated with PKA-dependent pathway via P1-like receptor but not with Ca2-dependent pathway via P2Y(2) receptor, and ATP degradation products do not contribute to this response. Further, stimulation of cells with ATP increa sed PKA activity. In addition, pretreatment with glybenclamide, an inhibito r of cystic fibrosis transmembrane conductance regulator, reduced the secon d peak of Isc induced by ATP but was without effect on that induced by UTP. Therefore, ATP stimulates glybenclamide-sensitive Cl- secretion, and this action is partly mediated by PKA-dependent pathway via P1-like receptor.