A novel and efficient tissue culture method - "stem-disc dome culture" - for producing virus-free garlic (Allium sativum L.)

Authors
Citation
M. Ayabe et S. Sumi, A novel and efficient tissue culture method - "stem-disc dome culture" - for producing virus-free garlic (Allium sativum L.), PL CELL REP, 20(6), 2001, pp. 503-507
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
20
Issue
6
Year of publication
2001
Pages
503 - 507
Database
ISI
SICI code
0721-7714(200109)20:6<503:ANAETC>2.0.ZU;2-F
Abstract
Using our previously reported stem-disc culture method as a basis, we have developed an efficient tissue culture method, "stem-disc dome (SD-Dome) cul ture", to eliminate viruses from infected garlic plants. Fifteen to 25 dome -shaped structures that formed on stem-disc explants from a single garlic c love were excised and maintained on phytohormone-free Linsmaier and Skoog m edium, as in the usual shoot-tip culture, These excised dome-shaped structu res grew independently into more than 5-cm-long shoots, rooted after 8 week s, and were successfully transplanted to soil. The resulting plants showed no viral symptoms on their leaves, even when cloves of garlic plants with s evere mosaic and yellow streak symptoms were used for the cultures. Examina tions of virus infection in three generations of the progenies using the di rect tissue blotting immunoassay and reverse transcription polymerase chain reaction showed that viruses were excluded during culture. Microscopic obs ervations of transverse sections of tissues in different developmental stag es during stem-disc culture - dome-shaped structures, shoot buds, and shoot s - suggested that the elimination of viruses may be correlated with the st age of development and organization of vascular-bundle structures.