DnaB, DnaD and DnaI proteins are components of the Bacillus subtilis replication restart primosome

Citation
C. Bruand et al., DnaB, DnaD and DnaI proteins are components of the Bacillus subtilis replication restart primosome, MOL MICROB, 42(1), 2001, pp. 245-255
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MOLECULAR MICROBIOLOGY
ISSN journal
0950-382X → ACNP
Volume
42
Issue
1
Year of publication
2001
Pages
245 - 255
Database
ISI
SICI code
0950-382X(200110)42:1<245:DDADPA>2.0.ZU;2-3
Abstract
Phenotypes of Bacillus subtilis priA mutants suggest that they are deficien t in the restart of stalled chromosomal replication forks. The presumed act ivity of PriA in the restart process is to promote the assembly of a multip rotein complex, the primosome, which functions to recruit the replication f ork helicase onto the DNA. We have proposed previously that three proteins involved in the initiation of replication at oriC in B. subtilis, DnaB, Dna D and Dnal, are components of the PriA primosome in this bacterium. However , the involvement of these proteins in replication restart has not yet been studied. Here, we describe dnaB mutations that suppress the phenotypes of B. subtilis priA mutants. In a representative mutant, the DnaC helicase is loaded onto single-stranded DNA in a PriA-independent, DnaD- and Dnal-depen dent manner. These observations confirm that DnaB, DnaD and Dnal are primos omal proteins in B. subtilis. Moreover, their involvement in the suppressio n of priA phenotypes shows that they participate in replication fork restar t in B. subtilis.