SEPH, a Cdc7p orthologue from Aspergillus nidulans, functions upstream of actin ring formation during cytokinesis

Citation
Ks. Bruno et al., SEPH, a Cdc7p orthologue from Aspergillus nidulans, functions upstream of actin ring formation during cytokinesis, MOL MICROB, 42(1), 2001, pp. 3-12
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MOLECULAR MICROBIOLOGY
ISSN journal
0950-382X → ACNP
Volume
42
Issue
1
Year of publication
2001
Pages
3 - 12
Database
ISI
SICI code
0950-382X(200110)42:1<3:SACOFA>2.0.ZU;2-P
Abstract
In the filamentous fungus, Aspergillus nidulans, multiple rounds of nuclear division occur before cytokinesis, allowing an unambiguous identification of genes required specifically for cytokinesis. As in animal cells, both an intact microtubule cytoskeleton and progression through mitosis are requir ed for actin ring formation and contraction. The sepH gene from A. nidulans was discovered in a screen for temperature-sensitive cytokinesis mutants. Sequence analysis showed that SEPH is 42% identical to the serine-threonine kinase Cdc7p from fission yeast. Signalling through the (S) under bar epta tion (I) under bar nitiation (N) under bar etwork (SIN), which includes Cdc 7p and the GTPase Spg1p, is emerging as a primary regulatory pathway used b y fission yeast to control cytokinesis. A similar group of proteins compris e the Mitotic Exit Network (MEN) in budding yeast. This is the first direct evidence for the existence of a functional SIN-MEN pathway outside budding and fission yeast. In addition to SEPH, potential homologues were also ide ntified in other fungi and plants but not in animal cells. Deletion of sepH resulted in a viable strain that failed to septate at any temperature. Int erestingly, quantitative analysis of the actin cytoskeleton revealed that s epH is required for construction of the actin ring. Therefore, SEPH is dist inct from its counterpart in fission yeast, in which SIN components operate downstream of actin ring formation and are necessary for ring contraction and later events of septation. We conclude that A. nidulans has components of a SIN-MEN pathway, one of which, SEPH, is required for early events duri ng cytokinesis.