Hypothalamic appetite-regulating neuropeptide mRNA levels in cachetic nudemice bearing human tumor cells

Citation
N. Nara-ashizawa et al., Hypothalamic appetite-regulating neuropeptide mRNA levels in cachetic nudemice bearing human tumor cells, METABOLISM, 50(10), 2001, pp. 1213-1219
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
METABOLISM-CLINICAL AND EXPERIMENTAL
ISSN journal
0026-0495 → ACNP
Volume
50
Issue
10
Year of publication
2001
Pages
1213 - 1219
Database
ISI
SICI code
0026-0495(200110)50:10<1213:HANMLI>2.0.ZU;2-M
Abstract
We previously reported that the human melanoma cell line, SEKI, induces sev ere weight loss in nude mice. In the present study, we examined the express ion of weight-regulating neuropeptide mRNAs in the hypothalamus of this can cer cachectic model by using a sensitive quantitative reverse transcriptase -polymerase chain reaction (RT-PCR) method and in situ hybridization. mRNA levels of neuropeptide Y (NPY) and corticotropin-releasing hormone (CRH) in the whole hypothalamus were elevated significantly in the SEKI mice as com pared with control mice. In situ hybridization showed that NPY and CRH mRNA were upregulated in the arcuate nucleus and the paraventricular nucleus, r espectively. There were no significant differences in melanin-concentrating hormone (MCH), orexin (OX), and cholecystokinin mRNA levels between the SE KI and control mice. These results suggest that the NPYergic system is func tioning in the rodent model of cancer cachexia; however, the role of the CR Hergic system in energy homeostasis remains to be elucidated. This is the f irst report of the hypothalamic neuropeptide response to each exia-inducing human cells. Copyright (C) 2001 by W.B. Saunders Company.