Apolipoprotein E and mimetic peptide initiate a calcium-dependent signaling response in macrophages

Citation
Uk. Misra et al., Apolipoprotein E and mimetic peptide initiate a calcium-dependent signaling response in macrophages, J LEUK BIOL, 70(4), 2001, pp. 677-683
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
JOURNAL OF LEUKOCYTE BIOLOGY
ISSN journal
0741-5400 → ACNP
Volume
70
Issue
4
Year of publication
2001
Pages
677 - 683
Database
ISI
SICI code
0741-5400(200110)70:4<677:AEAMPI>2.0.ZU;2-5
Abstract
Apolipoprotein E (ApoE) is a 34-kDa cholesterol transport protein that also possesses immunomodulatory properties. In this study, ive demonstrate that ApoE initiates a signaling cascade in murine peritoneal macrophages that l eads to increased production of inositol triphosphate with mobilization of intracellular Ca2+ stores. This cascade is inhibited by pretreatment with r eceptor-associated protein and Ni2+, and it is mediated by a pertussis toxi n-sensitive G protein. These properties are characteristic of signal transd uction induced via ligand binding to the cellular receptor, lipoprotein rec eptor-related protein. A peptide derived from the receptor-binding region o f ApoE also initiates signal transduction in a manner similar to that of th e intact protein, suggesting that this isolated region is sufficient for si gnal transduction. The ApoE-mimetic peptide competed for binding with the i ntact protein, confirming that they both interact with the same site. ApoE- dependent signal transduction might play a role in mediating the functional properties of this lipoprotein.