Microbial community dynamics and evaluation of bioremediation strategies in oil-impacted salt marsh sediment microcosms

Citation
Ds. Bachoon et al., Microbial community dynamics and evaluation of bioremediation strategies in oil-impacted salt marsh sediment microcosms, J IND MIC B, 27(2), 2001, pp. 72-79
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
1367-5435 → ACNP
Volume
27
Issue
2
Year of publication
2001
Pages
72 - 79
Database
ISI
SICI code
1367-5435(200108)27:2<72:MCDAEO>2.0.ZU;2-2
Abstract
Microbial community dynamics in wetlands microcosms emended! with commercia l products (surfactant, a biological agent, and nutrients) designed to enha nce bioremediation was followed for 3 months. The effectiveness of enhanced degradation was assessed by determining residual concentrations of individ ual petroleum hydrocarbons by GC/MS. The size and composition of the sedime nt microbial community was assessed using a variety of indices, including b acterial plate counts, MPNs, and DNA hybridizations with domain- and group- specific oligonucleotide probes. The addition of inorganic nutrients was th e most effective treatment for the enhancement of: oil degradation, resulti ng in marked degradation of petroleum alkanes and a lesser extent of degrad ation of aromatic oil constituents. The enhanced degradation was associated with increases in the amount of extractable microbial DNA and Streptomyces in the sediment, although not with increased viable counts (plate counts, MPN). Bacteria introduced with one of the proprietary products were still d etected in, the microcosms after 3 months, but were not a major quantitativ e constituent of the community. The biological product enhanced oil degrada tion relative to the control, but to a lesser extent than the nutrient addi tions alone. In contrast, application of the surfactant to the oil-impacted sediment decreased oil degradation.