Cross-genomic analysis of the translational systems of various organisms

Citation
K. Fujita et al., Cross-genomic analysis of the translational systems of various organisms, J IND MIC B, 27(3), 2001, pp. 163-169
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
1367-5435 → ACNP
Volume
27
Issue
3
Year of publication
2001
Pages
163 - 169
Database
ISI
SICI code
1367-5435(200109)27:3<163:CAOTTS>2.0.ZU;2-T
Abstract
We have characterized the genes encoding ribosomal proteins (r-proteins) as well as other translation-related factors of 15 eubacteria and four archae bacteria, and the genes for the mitochondrial r-proteins of Saccharomyces c erevisiae by using the complete genomic nucleotide sequence data of these o rganisms. In eubacteria, including two species of Mycoplasma, the operon st ructure of the r-protein genes is well conserved, while their relative orie ntation and chromosomal location are quite divergent. The operon structure of the r-protein genes in archaebacteria, on the other hand, is quite diffe rent from eubacteria and also among themselves. In addition, many archaebac terial r-proteins show similarity to rat cytoplasmic r-proteins. Nonetheles s, characteristic features of several genes encoding proteins of functional importance are well conserved throughout the bacterial species including a rchaebacteria, as well as in S. cerevisiae. We searched for the genes encod ing mitochondrial r-proteins in yeast by combining informatics and genetic experiments. Furthermore, we characterized some of the r-proteins genes by exchanging portions between Escherichia coli and S. cerevisiae and performe d functional analysis of some of the genes from different evolutionary poin ts of view. Our work may be extended towards phylogenetic analysis of organ isms producing secondary metabolites of various sorts.