Effect of essential amino acids on mouse embryo viability and ammonium production

Citation
M. Lane et al., Effect of essential amino acids on mouse embryo viability and ammonium production, J AS REPROD, 18(9), 2001, pp. 519-525
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Reproductive Medicine
Journal title
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
ISSN journal
1058-0468 → ACNP
Volume
18
Issue
9
Year of publication
2001
Pages
519 - 525
Database
ISI
SICI code
1058-0468(200109)18:9<519:EOEAAO>2.0.ZU;2-K
Abstract
Purpose. To examine the effect of essential amino acids concentrations on m ouse embryo development. Methods: Mouse embryos were cultured in medium with different concentration s of essential amino acids and development to the blastocyst stage and viab ility assessed. Ammonium production resulting from medium breakdown and ami no acid metabolism by embryos were also assessed. Results. Reducing the essential amino acid concentration significantly incr eased blastocyst development and cell numbers. Lowering the essential amino acid concentration decreased ammonium production in the medium. Conclusions. Culture media for the development of preimplantation embryos s hould have a reduced essential aml. no acid concentration to facilitate emb ryo development.