Purpose. To examine the effect of essential amino acids concentrations on m
ouse embryo development.
Methods: Mouse embryos were cultured in medium with different concentration
s of essential amino acids and development to the blastocyst stage and viab
ility assessed. Ammonium production resulting from medium breakdown and ami
no acid metabolism by embryos were also assessed.
Results. Reducing the essential amino acid concentration significantly incr
eased blastocyst development and cell numbers. Lowering the essential amino
acid concentration decreased ammonium production in the medium.
Conclusions. Culture media for the development of preimplantation embryos s
hould have a reduced essential aml. no acid concentration to facilitate emb