Cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF-ET

Citation
Ks. Lee et al., Cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF-ET, J AS REPROD, 18(9), 2001, pp. 490-498
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Reproductive Medicine
Journal title
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
ISSN journal
1058-0468 → ACNP
Volume
18
Issue
9
Year of publication
2001
Pages
490 - 498
Database
ISI
SICI code
1058-0468(200109)18:9<490:CCAAAI>2.0.ZU;2-T
Abstract
Purpose. Our purpose was to establish an evaluation system for oocyte quali ty based on the incidence of cumulus cells apoptosis and to examine the eff ect of coculture, using autologous cumulus cells, on the outcome of IVF-ET according to proliferative activities of helper cells and the incidence of cumulus cells apoptosis. Methods. Cumulus cell masses were collected from 91 mature oocytes among 33 0 oocytes retrieved fi-om a total of 34 IVF-ET cycles with tubal infertilit y and unexplained infertility. The incidence of apoptosis in cumulus cells was assessed by apoptosis detection kit fluorescein. On ovum pick tip, 2nd day embryos were cocultured with autologous cumulus cells. Prior to cocultu re, in vitro proliferative activity of cumulus cells was evaluated. Results. Cumulus cells from patient groups over 40 years old had a signific antly increased apoptosis incidence, a lower fertilization rate, and the de creased number of oocytes retrieved compared to the other age groups (P < . 05). The incidence of cumulus cells apoptosis was significantly lower when the number of oocytes retrieved was 5 or less (P < .05). Cumulus cells from fertilized oocytes (0.43 +/- 0.07%) and those from patients who became pre gnant (0.44 +/- 0.11%) following IVF-ET showed a significantly lower incide nce of apoptosis compared to those of unfertilized oocytes (1.80 +/- 0.35%; , P < .001) and the nonpregnant group (0.81 +/- 0.10%; P < .05). Embryo qua lity also had a negative correlation with the incidence of cumulus cells ap optosis. Coculture of fertilized oocytes with cumulus cells with high proli ferative activity resulted in improved rates of implantation and pregnancy compared to that with poor active cumulus cells. No significant difference was found between the in vitro proliferative activity of cumulus cells and the incidence of cumulus cells apoptosis (P < .063). Conclusions: The age of women might influence the incidence of apoptosis in cumulus cells, and the increased incidence of apoptosis is associated with the number of oocytes retrieved, the fertilization rate, and the pregnancy outcome following IVF-ET These results suggest that the incidence of cumul us cells apoptosis can be used in predicting oocyte quality., outcome of IV F-ET, and age-related decline in fertility.