The pattern of gene expression in mouse Gr-1(+) myeloid progenitor cells

Citation
Jj. Chen et al., The pattern of gene expression in mouse Gr-1(+) myeloid progenitor cells, GENOMICS, 77(3), 2001, pp. 149-162
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENOMICS
ISSN journal
0888-7543 → ACNP
Volume
77
Issue
3
Year of publication
2001
Pages
149 - 162
Database
ISI
SICI code
0888-7543(200110)77:3<149:TPOGEI>2.0.ZU;2-Z
Abstract
To understand the pattern of gene expression in mouse myeloid progenitor ce lls, we carried out a genome-wide analysis of gene expression in mouse bone marrow Gr-1(+) cells using SAGE and GLGI techniques. We identified 22,033 unique SAGE tags with quantitative information from 73,869 collected SAGE t ags. Among these unique tags, 64% match known sequences, including many gen es important for myeloid differentiation, and 36% have no matches to known sequences and are likely to represent novel genes. We compared the expressi on of mouse Gr-1(+) and human CD15(+) myeloid progenitor cells and showed t hat the pattern of gene expression of these two cell populations had some s imilarities. We also compared the expression of mouse Gr-1(+) myeloid proge nitor cells with that of mouse brain tissue and found a highly tissue-speci fic manner of gene expression in these two samples. Our data provide a basi s for studying altered gene expression in myeloid disorders using mouse mod els.