A comprehensive collection of mouse zinc finger motifs compiled by molecular indexing

Citation
R. Yamashita et al., A comprehensive collection of mouse zinc finger motifs compiled by molecular indexing, GENE, 274(1-2), 2001, pp. 101-110
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENE
ISSN journal
0378-1119 → ACNP
Volume
274
Issue
1-2
Year of publication
2001
Pages
101 - 110
Database
ISI
SICI code
0378-1119(20010822)274:1-2<101:ACCOMZ>2.0.ZU;2-R
Abstract
The C2H2 zinc finger motif found in many transcription factors is thought t o be for nucleic acid binding and/or dimerization. Nearly 1% of eukaryote g enes are estimated to encode this motif. We investigated the gene family en coding this motif in the Mus musculus mRNA by molecular indexing, a techniq ue used to select a subpopulation of cDNA by ligation of adapters to cDNA f ragments digested by a class HS restriction enzyme(s). In place of oligo-dT primers in the original method, a polymerase chain reaction primer designe d based on the conserved sequence of the C2H2 zinc finger protein stranded cDNA was made from various mouse tissue mRNAs, digested with FokI and BsmAI , and joined with adapters. Amplification of the cDNA with an adapter prime r and zinc finger-specific primer yielded products enriched in zinc finger protein genes. Fragments were separated by subsequent denaturing polyacryla mide gel electrophoresis, and characterized by DNA sequencing. Consequently , 259 C2H2 zinc finger motif sequences were obtained, among which 166 were novel. Combined with the reported sequences, these mouse motif sequences we re compared with those of other species such as Saccharomyces cerevisiae an d Caenorhabditis elegans. Some of the amino acids in the motif sequence sho wed strong bias among species. Most of the novel sequences were supposed to be DNA-binding according to the surface potential of predicted tertiary st ructures. (C) 2001 Elsevier Science B.V. All rights reserved.