Mouse Atp6f, the gene encoding the 23-kDa proteolipid of vacuolar proton translocating ATPase

Citation
Gh. Sun-wada et al., Mouse Atp6f, the gene encoding the 23-kDa proteolipid of vacuolar proton translocating ATPase, GENE, 274(1-2), 2001, pp. 93-99
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENE
ISSN journal
0378-1119 → ACNP
Volume
274
Issue
1-2
Year of publication
2001
Pages
93 - 99
Database
ISI
SICI code
0378-1119(20010822)274:1-2<93:MATGET>2.0.ZU;2-8
Abstract
The 23-kDa proteolipid subunit of mouse vacuolar-type proton-translocating ATPase (V-ATPase) was predicted to be a hydrophobic polypeptide of 205 amin o acid residues with five putative transmembrane segments. It exhibits sequ ence similarity to Vma16p of Saccharomyces cerevisiae and vha-4 of Caenorha bdittis elegans (83 and 84%, respectively). Southern blot analysis indicate d that the proteolipid is encoded by a single gene, Atp6f, in the mouse gen ome. Atp6f was mapped to approximately 55 cM on chromosome 4, and its genom ic organization is similar to that of the human gene: 8 exons separated by 7 introns, with boundaries matching the GT-AG rule. RNA blotting demonstrat ed that Atp6f is transcribed as 1.0- and 1.8-kb mRNAs in multiple tissues t o varying degrees. The major transcription initiation sites are at -13 and -58 bp upstream of the translation initiation codon. The epitope-tagged 23- kDa protoelipid was localized in endomembrane organelles in CHO cells, as e xpected for a component of a vacuolar-type proton pump. (C) 2001 Elsevier S cience B.V. All rights reserved.