Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4

Citation
Bj. Feys et al., Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4, EMBO J, 20(19), 2001, pp. 5400-5411
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO JOURNAL
ISSN journal
0261-4189 → ACNP
Volume
20
Issue
19
Year of publication
2001
Pages
5400 - 5411
Database
ISI
SICI code
0261-4189(20011001)20:19<5400:DIBTAD>2.0.ZU;2-M
Abstract
The Arabidopsis EDS1 and PAD4 genes encode lipase-like proteins that functi on in resistance (R) gene-mediated and basal plant disease resistance. Phen otypic analysis of eds] and pad4 null mutants shows that EDS1 and PAD4 are required for resistance conditioned by the same spectrum of R genes but ful fil distinct roles within the defence pathway. EDS1 is essential for elabor ation of the plant hypersensitive response, whereas EDS1 and PAD4 are both required for accumulation of the plant defence-potentiating molecule, salic ylic acid. EDS1 is necessary for pathogen-induced PAD4 mRNA accumulation, w hereas mutations in PAD4 or depletion of salicylic acid only partially comp romise EDS1 expression. Yeast two-hybrid analysis reveals that EDS1 can dim erize and interact with PAD4. However, EDS1 dimerization is mediated by dif ferent domains to those involved in EDS1-PAD4 association. Co-immunoprecipi tation experiments show that EDS1 and PAD4 proteins interact in healthy and pathogen-challenged plant cells. We propose two functions for EDS1. The fi rst is required early in plant defence, independently of PAD4. The second r ecruits PAD4 in the amplification of defences, possibly by direct EDS1-PAD4 association.