Comparison of protein adsorption patterns onto differently charged hydrophilic superparamagnetic iron oxide particles obtained in vitro and ex vivo

Citation
K. Lind et al., Comparison of protein adsorption patterns onto differently charged hydrophilic superparamagnetic iron oxide particles obtained in vitro and ex vivo, ELECTROPHOR, 22(16), 2001, pp. 3514-3521
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
22
Issue
16
Year of publication
2001
Pages
3514 - 3521
Database
ISI
SICI code
0173-0835(200110)22:16<3514:COPAPO>2.0.ZU;2-S
Abstract
Protein adsorption patterns of superparamagnetic iron oxides (SPIO) were ev aluated by two-dimensional electrophoresis (2-DE) after in vitro incubation of the particles in plasma or serum. SPIO particles having positive (MKK 1 211), negative (MKA 1211), or neutral (MKG 1411) charge were used. Protein adsorption patterns of different charged SPIO particles acquired in vitro a nd recollected 5 min after intravenous injection into rats (ex vivo) were c ompared. For the uncharged MKG 1411 particles, the differences of protein a dsorption patterns were negligible and only minor differences were found fo r the negatively charged MKA 1211 and positively charged MKK 1211 particles . A good correlation between in vitro and ex vivo data could be shown. For the evaluation of protein adsorption patterns of SPIO particles determining organ distribution and allowing estimation of site-specific delivery (drug targeting), the currently used protocol for 2-DE analysis could be confirm ed.