High-throughput characterization for organic pollutants in environmental waters using a capillary electrophoresis chip

Citation
S. Wakida et al., High-throughput characterization for organic pollutants in environmental waters using a capillary electrophoresis chip, ELECTROPHOR, 22(16), 2001, pp. 3505-3508
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
22
Issue
16
Year of publication
2001
Pages
3505 - 3508
Database
ISI
SICI code
0173-0835(200110)22:16<3505:HCFOPI>2.0.ZU;2-A
Abstract
To evaluate organic pollution in water, we did preliminarily studies on hig h-throughput characterization of organic pollution in water using microchip -based capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection. The applied voltage was investigated to control the gated valve injection and CE separation for conventional cross type microchips using a self-made personal computer (PC)-based controller as the voltage supply. W e obtained high-throughput data for the reproducible separation of fluoresc ein isothiocyanate (FITC)-labeled river-water samples using a zwitter-ion b ased buffer solution to avoid adsorption of the labeled sample onto the cha nnel of a microchip made from quartz glass. We used real samples from the H ino River that flows into Lake Biwa, from ten sampling points and obtained several reproducible peaks in different separation patterns for each sample within 2 min. We successfully demonstrated high-throughput characterizatio n of dissolved organic carbon (DOC) in environmental water using the microc hip.