Reduced expression of focal adhesion kinase in liver metastases compared with matched primary human colorectal adenocarcinomas

Citation
M. Ayaki et al., Reduced expression of focal adhesion kinase in liver metastases compared with matched primary human colorectal adenocarcinomas, CLIN CANC R, 7(10), 2001, pp. 3106-3112
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
CLINICAL CANCER RESEARCH
ISSN journal
1078-0432 → ACNP
Volume
7
Issue
10
Year of publication
2001
Pages
3106 - 3112
Database
ISI
SICI code
1078-0432(200110)7:10<3106:REOFAK>2.0.ZU;2-T
Abstract
The focal adhesion kinase (FAK) is implicated in integrin-mediated signal t ransduction pathways used in cell adhesion, cell motility, apoptosis, and a nchorage-independent growth. Because cancer invasion and metastasis are tho ught to be associated with alterations in cellular adhesive and motile prop erties, we studied the expression of four focal adhesion proteins including FAK in matched samples of human normal colorectal mucosa (N), primary colo rectal adenocarcinomas (T) and liver metastases (NI) from 10 patients by We stern blot analysis. This gave us the advantage of directly comparing level s of focal adhesion protein expression within the same genetic background. Average FAK expression level was significantly higher in T than in N and it was significantly lower in NI than in T. Average paxillin expression level was also significantly higher in T than in N, but it was not significantly different between T and M. Similar results were obtained by immunohistoche mical analyses of FAK and paxillin expression. Average vinculin and talin e xpression levels showed no significant differences among these three sample s (N, T, and NI). These data demonstrate that the FAK expression level incr eases in primary tumors compared with normal mucosa and decreases in liver metastases to the level of normal mucosa in the majority of human colorecta l adenocarcinomas. Up- and downregulation of FAK protein expression observe d in this study may have a profound effect on the signal transduction.