Automated color correction method for Vibrio fischeri toxicity test. Comparison of standard and kinetic assays

Citation
J. Lappalainen et al., Automated color correction method for Vibrio fischeri toxicity test. Comparison of standard and kinetic assays, CHEMOSPHERE, 45(4-5), 2001, pp. 635-641
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Environment/Ecology
Journal title
CHEMOSPHERE
ISSN journal
0045-6535 → ACNP
Volume
45
Issue
4-5
Year of publication
2001
Pages
635 - 641
Database
ISI
SICI code
0045-6535(200111)45:4-5<635:ACCMFV>2.0.ZU;2-L
Abstract
We demonstrate in this study that the toxicity of solid and highly colorful samples can be measured with kinetic bioassay using luminescent bacterium Vibrio fischeri. The Flash assay, named after the test protocol, is perform ed with a tube luminometer. In this method, each sample acts as a reference for itself, and therefore, the color correction is possible with minimal h ands-on-time. The bacteria are dispensed into the sample and the signal is recorded continuously. The maximum signal received after immediately dispen sing is compared to the signal after an incubation period. With many chemic als, the toxic effects are obtained after a very short contact time. Howeve r, different chemicals have different modes of toxicity. Thus, kinetic data from sample analyses after 15 or 30 min for this bacterium gives an additi onal dimension for obtaining reliable results. The performance of the test was compared to the standardized photobacteria test protocol with reference chemicals. The repeatability of the test was excellent. The coefficient of variation was normally below 1% with 10 replicates. (C) 2001 Elsevier Scie nce Ltd. All rights reserved.