S. Fujioka et al., Angiogenesis in pancreatic carcinoma - Thymidine phosphorylase expression in stromal cells and intratumoral microvessel density as independent predictors of overall and relapse-free survival, CANCER, 92(7), 2001, pp. 1788-1797
BACKGROUND. Recently, the usefulness of intratumoral microvessel density (I
MD) and expression of several angiogenic factors as prognostic indicators h
ave been demonstrated in several human solid tumors.
METHODS. One hundred four patients with pancreatic ductal adenocarcinoma we
re examined retrospectively. The investigated clinicopathologic and immunoh
istologic data included staining for vascular endothelial growth factor (VE
GF), thymidine phosphorylase (TP), basic fibroblast growth factor (bFGF), C
D34 (for calculating IMD), p53, and Ki-67.
RESULTS. Multivariate analysis for both overall and relapse-free survival r
evealed two independent variables, IMD and TP staining in stromal cells (TP
s, P < 0.02). Whereas the frequency of hepatic metastasis was correlated si
gnificantly with cytoplasmic expression of TP or bFGF in tumor cells (TPc,
bFGFc), IMD, and p53 status, local recurrence was significantly more common
in patients with positive staining for TPs, bFGF in stromal cells (bFGFs),
and for the pM category (P < 0.05). TPc, bFGFc, vEGF, and p53 expression c
orrelated with IMD (P < 0.01), although TPs and bFGFs expression did not. v
EGF and IMD status correlated with p53 expression (P < 0.001), although TP,
bFGF, and Ki-67 status did not.
CONCLUSIONS. TPs expression and IMD were revealed to be valuable tools for
predicting overall and relapse-free survival in patients with pancreatic ad
enocarcinoma. Whereas TPc and bFGFc are likely to participate in hepatic me
tastasis by means of their angiogenic properties, TPs and bFGFs may be rela
ted to local tumor progression. Angiogenesis in human pancreatic carcinoma
may be dependent on vEGF, TP, and bFGF. p53 abnormality is likely to take p
art in VEGF-related angiogenesis. (C) 2001 American Cancer Society.