Polymorphisms in the coding region of mtDNA and effects on clinical outcome of unrelated bone marrow transplantation

Citation
Y. Ishikawa et al., Polymorphisms in the coding region of mtDNA and effects on clinical outcome of unrelated bone marrow transplantation, BONE MAR TR, 28(6), 2001, pp. 603-607
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Medical Research Diagnosis & Treatment
Journal title
BONE MARROW TRANSPLANTATION
ISSN journal
0268-3369 → ACNP
Volume
28
Issue
6
Year of publication
2001
Pages
603 - 607
Database
ISI
SICI code
0268-3369(200109)28:6<603:PITCRO>2.0.ZU;2-J
Abstract
The entire protein-coding region was divided into 45 fragments, separately amplified and analyzed for polymorphism by the PCR-SSCP (single-strand conf ormation polymorphism) method. The effect of polymorphism mismatching on th e clinical outcome of unrelated bone marrow transplantation was studied to clarify whether products from mtDNA become minor antigens. Variability in P CR-SSCP pattern combinations of the 45 fragments suggests that each individ ual has a different polymorphism combination in the protein-coding region i f all the coding regions were compared at the nucleotide sequence level. No nsynonymous polymorphisms were found at relatively high frequency in MTATP8 and MTND3. Both the polymorphisms with and without substitution matched th e peptide-binding motifs of HLA-A*0201. The effects of the polymorphism mat ching were retrospectively analyzed in 340 recipients transplanted with HLA -A, -B, -DRB1 allele-matched bone marrow from unrelated donors. There were no effects of polymorphism matching on the incidence of acute GVHD and cumu lative disease-free survival. These results suggest that polymorphisms whic h generate peptides, with and without substitutions, that bind the same HLA molecule hardly influence GVHD because the difference between the HLA-pept ide complexes is minute.