A role for membrane-type serine protease (MT-SP1) in intestinal epithelialturnover

Citation
S. Satomi et al., A role for membrane-type serine protease (MT-SP1) in intestinal epithelialturnover, BIOC BIOP R, 287(4), 2001, pp. 995-1002
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
287
Issue
4
Year of publication
2001
Pages
995 - 1002
Database
ISI
SICI code
0006-291X(20011005)287:4<995:ARFMSP>2.0.ZU;2-8
Abstract
Membrane type-serine protease 1 (MT-SP1) plays potential roles in the proce ss of invasion and metastasis of carcinomas. In the present study, we clone d a rat MT-SP1 cDNA and investigated the intestinal distribution and proteo lytic properties of the enzyme. By in situ hybridization we found the promi nent expression of the mRNA in the epithelial layer of the small intestinal upper villi and of the colon, where cells are loosely attached to the base ment membrane. When MT-SP1 was expressed in Caco-2, a colonic carcinoma cel l line, the protein was localized exclusively on the basolateral side. A se creted form of the enzyme produced in COS-1 cells digested fibronectin and laminin. These findings suggest that MT-SP1 participates in the control of intestinal epithelial turnover by regulating the cell-substratum adhesion. (C) 2001 Academic Press.