Infiltration of neutrophils following injection of apoptotic cells into the peritoneal cavity

Citation
R. Misawa et al., Infiltration of neutrophils following injection of apoptotic cells into the peritoneal cavity, APOPTOSIS, 6(6), 2001, pp. 411-417
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
APOPTOSIS
ISSN journal
1360-8185 → ACNP
Volume
6
Issue
6
Year of publication
2001
Pages
411 - 417
Database
ISI
SICI code
1360-8185(200112)6:6<411:IONFIO>2.0.ZU;2-P
Abstract
It has been assumed that apoptosis leads to no production of pro-inflammato ry cytokines or the production of anti-inflammatory cytokines in vivo, alth ough the response of macrophages following phagocytosis of apoptotic cells in vivo has not been examined. In this study we therefore examined the resp onse to apoptotic cells in vivo. Injection of apoptotic cells into the peri toneal cavity of mice led to transient neutrophil infiltration and concomit ant production of MIP-2, a mouse homologue of IL-8. Apoptotic cells were ph agocytosed by macrophages, as revealed on two-color flow cytometric analysi s and microscopic observation. When the mice were depleted of macrophages b y pretreatment with liposome-encapsulated dichloromethylene bisphosphonate, both neutrophil infiltration and MIP-2 production were significantly suppr essed, suggesting that macrophages are required for MIP-2 production in thi s in vivo response. These results support the hypothesis that extensive apo ptosis occurring rapidly may induce an inflammatory response in vivo.