Immunohistological study of infiltrated cells and cytokines in murine herpetic keratitis

Citation
T. Inoue et al., Immunohistological study of infiltrated cells and cytokines in murine herpetic keratitis, ACT OPHTH S, 79(5), 2001, pp. 484-487
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Optalmology
Journal title
ACTA OPHTHALMOLOGICA SCANDINAVICA
ISSN journal
1395-3907 → ACNP
Volume
79
Issue
5
Year of publication
2001
Pages
484 - 487
Database
ISI
SICI code
1395-3907(200110)79:5<484:ISOICA>2.0.ZU;2-7
Abstract
Purpose. To identify localization and kinetics of infiltrated cells and cyt okines in murine herpetic keratitis. Methods. HSV-1 was inoculated onto the scarified BALM corneas. At given tim es post infection (Pl), eyes were removed and studied immunohistochemically using monoclonal antibodies against several infiltrated cells and cytokine s. Results. Neutrophils and NK cells infiltrated as early as 1 day PI reaching a maximum number at 2 day PI in initial stage. gamma delta TCR positive ce lls were observed in the corneal stroma from I day PI to 8 day Pl. IL-2 and IFN-gamma were positive in the cell-infiltrated areas of the and stromal l esions, whereas IL-4 was negative throughout the experiment. Conclusion. Our results indicated that cytokine profile upon herpes infecti on on the cornea is Th1 dominant. Together with neutrophils in the early ph ase of infection, gamma delta positive T cells may play an additional role in protecting the cornea against incoming pathogens.