Purpose. To identify localization and kinetics of infiltrated cells and cyt
okines in murine herpetic keratitis.
Methods. HSV-1 was inoculated onto the scarified BALM corneas. At given tim
es post infection (Pl), eyes were removed and studied immunohistochemically
using monoclonal antibodies against several infiltrated cells and cytokine
Results. Neutrophils and NK cells infiltrated as early as 1 day PI reaching
a maximum number at 2 day PI in initial stage. gamma delta TCR positive ce
lls were observed in the corneal stroma from I day PI to 8 day Pl. IL-2 and
IFN-gamma were positive in the cell-infiltrated areas of the and stromal l
esions, whereas IL-4 was negative throughout the experiment.
Conclusion. Our results indicated that cytokine profile upon herpes infecti
on on the cornea is Th1 dominant. Together with neutrophils in the early ph
ase of infection, gamma delta positive T cells may play an additional role
in protecting the cornea against incoming pathogens.