Interleukin-1-induced subacromial synovitis and shoulder pain in rotator cuff diseases

Citation
M. Gotoh et al., Interleukin-1-induced subacromial synovitis and shoulder pain in rotator cuff diseases, RHEUMATOLOG, 40(9), 2001, pp. 995-1001
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Rheumatology
Journal title
RHEUMATOLOGY
ISSN journal
1462-0324 → ACNP
Volume
40
Issue
9
Year of publication
2001
Pages
995 - 1001
Database
ISI
SICI code
1462-0324(200109)40:9<995:ISSASP>2.0.ZU;2-3
Abstract
Objective. To determine the relationship between the expression of interleu kin-1 beta (IL-1 beta) and IL-1 receptor antagonists (IL-1ra) in the subacr omial bursa and shoulder pain in rotator cuff diseases. Methods. Synovial specimens were analysed using various methods including r everse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemist ry and in situ RT-PCR. Thirty-nine patients with rotator cuff diseases were candidates. The degree of their shoulder pain was evaluated using a visual analogue scale. Results. The mRNA expression levels of the cytokines were significantly cor related with the degree of pain [IL-1 beta: r=0.782; secreted IL-1ra (sIL-1 ra): r=0.756; intracellular IL-1ra (icIL-1ra): r=0.806, P <0.001, respectiv ely]. The combined results of immunohistochemistry and in situ RT-PCR analy sis indicated that both synovial lining and sublining cells produce IL-1 be ta, while synovial lining cells predominantly produce icIL-1ra and sublinin g cells secrete sIL-1ra. Conclusions. The differential regulation of the two forms of IL-1ra mRNAs m ay play an important role in shoulder pain in rotator cuff diseases, regula ting IL-1-induced subacromial synovitis.