Comparison of the effects of different antiviral treatments on the antioxidant systems of stroma-free hemoglobin

Citation
J. Hirayama et al., Comparison of the effects of different antiviral treatments on the antioxidant systems of stroma-free hemoglobin, PHOTOCHEM P, 74(3), 2001, pp. 461-464
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PHOTOCHEMISTRY AND PHOTOBIOLOGY
ISSN journal
0031-8655 → ACNP
Volume
74
Issue
3
Year of publication
2001
Pages
461 - 464
Database
ISI
SICI code
0031-8655(200109)74:3<461:COTEOD>2.0.ZU;2-4
Abstract
The effect of virus inactivation by 1,9-dimethylmethylene blue (DMMB) photo treatment, methylene blue (MB) phototreatment or heat on the activities of antioxidant systems of stroma-free hemoglobin (SFH) was studied. DMMB photo inactivated human immunodeficiency virus by >3.69 log(10) under conditions that inactivated 3.33 log(10), of vesicular stomatitis virus (VSV). Under c onditions which inactivated VSV by 6.10 log(10) (1.37 J/cm(2) irradiation a nd 2 muM DMMB), there was little change in the methemoglobin (Met-Hb) forma tion, concentration of reduced glutathione (GSH), or superoxide dismutase ( SOD), catalase (CAT) or glutathione peroxidase (GPX) activities. However, t he activity of glutathione reductase (GR) was decreased by 77%. Under condi tions that inactivated VSV by 5.69 log(10) (1.37 J/cm(2) irradiation and 24 KM MB) there was little effect of MB phototreatment on SOD, CAT, GPX and G SH activities. However, GR activity was decreased by 74% and Met-Hb content reached 3.98%. Under conditions that inactivated VSV by more than 6.20 log (10) (60 degreesC for 2 min), virucidal heat treatment resulted in 27% Met- Hb formation and decreased GPX activity by 43%. No significant decline in S OD, CAT or GR activities or GSH concentration was observed. These results s uggest that, compared with heat treatment and MB phototreatment, virucidal DMMB treatment preserves not only the oxidative state of hemoglobin but als o the antioxidant systems against superoxide and hydrogen peroxide, althoug h the reduced GR activity may limit the quenching capacity of antioxidants in DMMB-treated SFH.