Recombineering: A powerful new tool for mouse functional genomics

Citation
Ng. Copeland et al., Recombineering: A powerful new tool for mouse functional genomics, NAT REV GEN, 2(10), 2001, pp. 769-779
Citations number
79
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
NATURE REVIEWS GENETICS
ISSN journal
1471-0056 → ACNP
Volume
2
Issue
10
Year of publication
2001
Pages
769 - 779
Database
ISI
SICI code
1471-0056(200110)2:10<769:RAPNTF>2.0.ZU;2-4
Abstract
Highly efficient phage-based Escherichia coli homologous recombination syst ems have recently been developed that enable genomic DNA in bacterial artif icial chromosomes to be modified and subcloned, without the need for restri ction enzymes or DNA ligases. This new form of chromosome engineering, term ed recombinogenic engineering or recombineering, is efficient and greatly d ecreases the time it takes to create transgenic mouse models by traditional means, Recombineering also facilitates many kinds of genomic experiment th at have otherwise been difficult to carry out, and should enhance functiona l genomic studies by providing better mouse models and a more refined genet ic analysis of the mouse genome.