The transfer of choline from the host to the bacterial cell surface requires glpQ in Haemophilus influenzae

Citation
X. Fan et al., The transfer of choline from the host to the bacterial cell surface requires glpQ in Haemophilus influenzae, MOL MICROB, 41(5), 2001, pp. 1029-1036
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MOLECULAR MICROBIOLOGY
ISSN journal
0950-382X → ACNP
Volume
41
Issue
5
Year of publication
2001
Pages
1029 - 1036
Database
ISI
SICI code
0950-382X(200109)41:5<1029:TTOCFT>2.0.ZU;2-L
Abstract
Haemophilus influenzae incorporates choline obtained from environmental sou rces onto its lipopolysaccharide as phosphorylcholine (ChoP). The decoratio n of the bacterial surface with ChoP contributes to pathogenesis by allowin g for mimicry of the host. As the main reservoir for choline in the host is phosphatidylcholine, we tested whether other choline-containing molecules associated with eukaryotic membranes could provide an alternative source of choline. H. influenzae was able to use glycerophosphorylcholine (GPC), an abundant degradation product of phospholipids, as efficiently as free choli ne. Utilization of GPC required glpQ, which expresses an enzyme with glycer ophosphodiester phosphodiesterase activity. In the absence of free choline, this gene was required for adherent H. influenzae to obtain choline direct ly from epithelial cells in culture. GlpQ therefore allows choline to be tr ansferred from the host to the bacterial cell surface.