Quantitative analysis of expression of two circadian clock-controlled geneclusters coding for the bidirectional hydrogenase in the cyanobacterium Synechococcus sp PCC7942

Citation
O. Schmitz et al., Quantitative analysis of expression of two circadian clock-controlled geneclusters coding for the bidirectional hydrogenase in the cyanobacterium Synechococcus sp PCC7942, MOL MICROB, 41(6), 2001, pp. 1409-1417
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MOLECULAR MICROBIOLOGY
ISSN journal
0950-382X → ACNP
Volume
41
Issue
6
Year of publication
2001
Pages
1409 - 1417
Database
ISI
SICI code
0950-382X(200109)41:6<1409:QAOEOT>2.0.ZU;2-S
Abstract
Hydrogen metabolism is of central interest in cyanobacterial research becau se of its potential applications. The gene expression and physiological rol e of the cyanobacterial bidirectional NAD(P)(+)-reducing hydrogenase are po orly understood. Transcription rates of hoxEF and hoxUYH encoding this enzy me have been studied in Synechococcus sp. PCC7942. P-hoxU activity was abou t three times higher than that Of P-hoxE. Circadian phasing of both promote rs was found to be synchronous and influenced expression levels by at least one order of magnitude. This is the first demonstration of circadian contr ol of gene expression for any hydrogenase. For the majority of P-hoxU-drive n messages, transcription presumably terminates between hoxU and hoxH. Bein g part of a polycistronic hoxUYHW... operon, hoxW, encoding a protease invo lved in C-terminal processing of the hydrogenase large-subunit HoxH, is mai nly expressed by its own promoter, P-hoxW. The complex transcript formation may be a key feature for controlling bidirectional hydrogenase expression in vivo.