Convenient method for the determination of mono-, di-, and triacylglycerine distribution including regioisomers by C-13 nuclear magnetic resonance spectroscopy

Citation
K. Hori et al., Convenient method for the determination of mono-, di-, and triacylglycerine distribution including regioisomers by C-13 nuclear magnetic resonance spectroscopy, J JPN SOC F, 48(9), 2001, pp. 650-655
Citations number
2
Language
GIAPPONESE
art.tipo
Article
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
ISSN journal
1341-027X → ACNP
Volume
48
Issue
9
Year of publication
2001
Pages
650 - 655
Database
ISI
SICI code
1341-027X(2001)48:9<650:CMFTDO>2.0.ZU;2-5
Abstract
By using carbon-13 (C-13) nuclear magnetic resonance (NMR) spectrometer, we have developed a easy quantitative method for determining the molar ratio of partial acylglycerines <1,2diacylglycerine (DG), 1,3-DG, 1-monoacylglyce rine (MG), and 2-MG > to triacylglycerine (TG) and/or glycerin. For refinem ent of some measurement conditions, we set the five kinds of palmitoyl acyl glycerines (TG, 1,2-DG, 1,3-DG, I-MG, and 2-MG) and glycerine as model comp ounds. After examining the solubility of these model compounds, we decided that pyridine-d(G) was suitable as an NMR measuring solvent. We also set th e delay time of the pulse repeating at 15s, which is five times longer than the longest spin-lattice relaxation times (Ti) of proton (H-1) NMR signals of the glycerine moieties protons and C-13 NMR signals of the glycerine mo ieties carbons. Good agreement was observed between the result of the C-13 NMR and that of the reverse-phase high performance liquid chromatography (H PLC) by the model compounds, When the characteristic peaks are quantitative ly measured in C-13 NMR of the commercial diacylglycerins product, each mol ar ratio of the derivatives, such as TG, 1,2-DG and 1,3-DG, can easily be d etermined.