The second serine acetyltransferase, bacterial-type O-acetylserine (thiol)lyase and eukaryotic-type O-acetylserine (thiol) lyase from the primitive red alga Cyanidioschyzon merolae
K. Toda et al., The second serine acetyltransferase, bacterial-type O-acetylserine (thiol)lyase and eukaryotic-type O-acetylserine (thiol) lyase from the primitive red alga Cyanidioschyzon merolae, J PLANT RES, 114(1115), 2001, pp. 291-300
A second serine acetyltransferase (SAT) gene and two O-acetylserine (thiol)
lyase (OASTL) genes were isolated by screening a genomic library of the pr
imitive red alga Cyanidioschyzon merolae and named cmSAT2, and cmOASTL1 and
cmOASTL2, respectively. cmSAT2 encoded a polypeptide of 406 amino acids. c
mSAT2 was encoded on chromosome IX. cmOASTL1 and cmOASTL2 encoded for polyp
eptides of 389 and 390 amino acids, respectively. A molecular phylogenic tr
ee of the amino acid sequences suggested that cmOASTL1 belongs to the group
of eukaryotic plant OASTL while cmOASTL2 belongs to the bacterial type OAS
TL. cmOASTL1 and cmOASTL2 were encoded on chromosomes XVII and VIII, respec
tively. In Northern blot analyses, the probes for cmOASTL1 and cmOASTL2 hyb
ridized with 1.4 kb and 1.3 kb bands, respectively. The identity of cmOASTL
1 and cmOASTL2 was confirmed by genetic complementation in an OASTL- defici
ent mutant of Escherichia coil NK3 using sulfate or sulfide as a sole sourc
e of sulfur.