The second serine acetyltransferase, bacterial-type O-acetylserine (thiol)lyase and eukaryotic-type O-acetylserine (thiol) lyase from the primitive red alga Cyanidioschyzon merolae

Citation
K. Toda et al., The second serine acetyltransferase, bacterial-type O-acetylserine (thiol)lyase and eukaryotic-type O-acetylserine (thiol) lyase from the primitive red alga Cyanidioschyzon merolae, J PLANT RES, 114(1115), 2001, pp. 291-300
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF PLANT RESEARCH
ISSN journal
0918-9440 → ACNP
Volume
114
Issue
1115
Year of publication
2001
Pages
291 - 300
Database
ISI
SICI code
0918-9440(200109)114:1115<291:TSSABO>2.0.ZU;2-V
Abstract
A second serine acetyltransferase (SAT) gene and two O-acetylserine (thiol) lyase (OASTL) genes were isolated by screening a genomic library of the pr imitive red alga Cyanidioschyzon merolae and named cmSAT2, and cmOASTL1 and cmOASTL2, respectively. cmSAT2 encoded a polypeptide of 406 amino acids. c mSAT2 was encoded on chromosome IX. cmOASTL1 and cmOASTL2 encoded for polyp eptides of 389 and 390 amino acids, respectively. A molecular phylogenic tr ee of the amino acid sequences suggested that cmOASTL1 belongs to the group of eukaryotic plant OASTL while cmOASTL2 belongs to the bacterial type OAS TL. cmOASTL1 and cmOASTL2 were encoded on chromosomes XVII and VIII, respec tively. In Northern blot analyses, the probes for cmOASTL1 and cmOASTL2 hyb ridized with 1.4 kb and 1.3 kb bands, respectively. The identity of cmOASTL 1 and cmOASTL2 was confirmed by genetic complementation in an OASTL- defici ent mutant of Escherichia coil NK3 using sulfate or sulfide as a sole sourc e of sulfur.