Preliminary analysis and annotation of the partial genome sequence of Francisella tularensis strain Schu 4

Citation
Rg. Prior et al., Preliminary analysis and annotation of the partial genome sequence of Francisella tularensis strain Schu 4, J APPL MICR, 91(4), 2001, pp. 614-620
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF APPLIED MICROBIOLOGY
ISSN journal
1364-5072 → ACNP
Volume
91
Issue
4
Year of publication
2001
Pages
614 - 620
Database
ISI
SICI code
1364-5072(200110)91:4<614:PAAAOT>2.0.ZU;2-1
Abstract
Francisella tularensis, the aetiological agent of tularemia, is an importan t pathogen throughout much of the Northern hemisphere. We have carried out sample sequencing of its genome in order to gain a greater insight into thi s organism about which very little is known, especially at the genetic leve l. Nucleotide sequence data from a genomic DNA shotgun library of the virul ent F. tularensis strain Schu 4 has been partially assembled to provide 1.8 3 Mb of the genome sequence. A preliminary analysis of the F. tularensis ge nome sequence has been performed and the data compared with 20 fully sequen ced and annotated bacterial genomes. Plasmid-encoded genes, previously isol ated from low virulence strains of F. tularensis, were not identified. A to tal of 1289 potential coding ORFs were identified in the data set., An anal ysis of this data revealed 413 ORFs which would encode proteins with no hom ology to known proteins. ORFs which could encode proteins involved in amino acid and purine biosynthesis were also identified. These biosynthetic path ways provide targets for the construction of a defined attenuated mutant of F. tularensis for use as a vaccine against tularemia.