Separation of ritalin racemate and its by-product racemates by capillary electrophoresis

Citation
Wx. Huang et al., Separation of ritalin racemate and its by-product racemates by capillary electrophoresis, ELECTROPHOR, 22(15), 2001, pp. 3226-3231
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
22
Issue
15
Year of publication
2001
Pages
3226 - 3231
Database
ISI
SICI code
0173-0835(200109)22:15<3226:SORRAI>2.0.ZU;2-4
Abstract
Ritalin, [(+)-threo]methylphenidate hydrochloride, is a chiral drug substan ce with two chiral centers. The drug substance may contain three pairs of e nantiomers, [(+)-threo], [(-)-threo], [(+)-erythro] and [(-)-erythro] isome rs, and its degradation products, threo-ritalinic acid racemate. Determinat ion of the optical purity of ritalin drug substance and the amount of its b y-product isomers is a critical step in the single-isomer drug development. In order to efficiently recognize the three pairs of enantiomers by one me thod, capillary electrophoresis (CE) was employed for the separation. The t hree pairs of enantiomers in CE showed different enantioselectivities with eight different types of CDs. Only 2,6-di-o-methyl-beta -cyclodextrin (DM-b eta -CD) and carboxymethyl-beta -cyclodextrin (CM-beta -CD) showed enantios electivity to all these pairs of enantiomers. With respect to separation re solution and efficiency, DM-beta -CD was chosen as the chiral selector. For optimization of the separation conditions, the concentration of DM-beta -C D, pH of the buffer solution, and temperature of the capillary were further studied.