Visualization of mPer1 transcription in vitro: NMDA induces a rapid phase shift of mPer1 gene in cultured SCN

Citation
M. Asai et al., Visualization of mPer1 transcription in vitro: NMDA induces a rapid phase shift of mPer1 gene in cultured SCN, CURR BIOL, 11(19), 2001, pp. 1524-1527
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
CURRENT BIOLOGY
ISSN journal
0960-9822 → ACNP
Volume
11
Issue
19
Year of publication
2001
Pages
1524 - 1527
Database
ISI
SICI code
0960-9822(20011002)11:19<1524:VOMTIV>2.0.ZU;2-9
Abstract
Many physiological and behavioral phenomena are controlled by an internal, self-sustaining oscillator with a periodicity of approximately 24 hr. In ma mmals, the principal oscillator resides in the suprachiasmatic nucleus (SCN ). A light pulse during the subjective night causes a phase shift of the ci rcadian rhythm via direct glutamatergic retinal afferents to the SCN [1]. A long with the accepted theoretical models of the clock, it is suggested tha t behavioral resetting of mammals is completed within 2 hr [2]; however, th e molecular mechanism has not been elucidated. Here, we show the realtime i mage of the transcription of the circadian-clock gene mPer1 in the cultured SCN by using the transgenic. mice that carry a luciferase reporter gene un der the control of the mPer1 promoter [3]. The real-time image demonstrates that the mPer1 promoter activity oscillates robustly in a circadian manner and that this promoter activity is reset rapidly (within 2-3 hr) when a ph ase shift occurs.