Isolation and expression of cathepsin B cDNA in hemocytes during metamorphosis of Bombyx mori

Citation
Ys. Xu et H. Kawasaki, Isolation and expression of cathepsin B cDNA in hemocytes during metamorphosis of Bombyx mori, COMP BIOC B, 130(3), 2001, pp. 393-399
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN journal
1096-4959 → ACNP
Volume
130
Issue
3
Year of publication
2001
Pages
393 - 399
Database
ISI
SICI code
1096-4959(200110)130:3<393:IAEOCB>2.0.ZU;2-T
Abstract
By using RT-PCR and 5'- and 3'-RACE methods, we isolated cathepsin B cDNA f rom hemocytes of Bombyx mori. The predicted open reading frame encoded 337 amino acids with 54% identity with the human cathepsin B, and 55% identity with the 29-kDa proteinase of the blowfly. Three active sites characteristi c for cathepsin B were conserved in the deduced amino acid sequences of BmC tB cDNA at positions Cys-111, His-280 and Asn-300. Northern analysis identi fied a 1.3-kb mRNA. Expression of BmCtB was observed in the hemocytes from the day of wandering and was strongest at day 1 pupa. With in situ hybridiz ation, strong signals were observed in the granular cells and plasmatocytes of day 0 pupa, but not in those of day 5 of the fifth larval instar. The r esults indicate another function of cathepsin B and insect hemocyte during metamorphosis. (C) 2001 Elsevier Science Inc. All rights reserved.