Dehydroepiandrosterone (DHEA) stimulates glucose uptake in rat adipocytes:activation of phospholipase D

Citation
M. Ishizawa et al., Dehydroepiandrosterone (DHEA) stimulates glucose uptake in rat adipocytes:activation of phospholipase D, COMP BIOC B, 130(3), 2001, pp. 359-364
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN journal
1096-4959 → ACNP
Volume
130
Issue
3
Year of publication
2001
Pages
359 - 364
Database
ISI
SICI code
1096-4959(200110)130:3<359:D(SGUI>2.0.ZU;2-7
Abstract
We examined the effect of dehydroepiandrosterone (DHEA) on glucose uptake a nd phospholipase D (PLD) activation in rat adipocytes. DHEA (1 muM) provoke d a twofold increase in [H-3]2-deoxyglucose (DG) uptake for 30 min. Incorpo ration of [H-3]-glycerol into diacylglycerol was increased 150% above basal level for 20 min after stimulation with 1 muM DHEA. DHEA increased PLD act ivity, measured by the incorporation into [H-3]phosphatidylethanol in [H-3] palmitate labelled rat adipocytes, or by [H-3]choline release in [methyl-H- 3]choline labeled rat adipocytes. Our results suggest that DHEA stimulates glucose uptake with activation of PLD in rat adipocytes. (C) 2001 Elsevier Science Inc. All rights reserved.