Kinetics of aerobic cometabolism of chlorinated solvents

Citation
L. Alvarez-cohen et Ge. Speitel, Kinetics of aerobic cometabolism of chlorinated solvents, BIODEGRADAT, 12(2), 2001, pp. 105-126
Citations number
123
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIODEGRADATION
ISSN journal
0923-9820 → ACNP
Volume
12
Issue
2
Year of publication
2001
Pages
105 - 126
Database
ISI
SICI code
0923-9820(2001)12:2<105:KOACOC>2.0.ZU;2-T
Abstract
The objectives of this paper are to review the wide range of kinetic models that have been introduced to describe the cometabolic oxidation of chlorin ated solvents, to compare modeling approaches and associated experimental d ata, and to discuss knowledge gaps in the general topic of cometabolism kin etics. To begin, a brief description of the mechanism of oxygenase enzyme m etabolism and its qualitative effects on cometabolic degradation kinetics i s given. Next, a variety of kinetic expressions that have been used to desc ribe cometabolism, ranging from adaptations of simple metabolic relationshi ps to the development of complex equations that account for intracellular c oncentrations of key reaction species, are presented. A large number of kin etic coefficients published for a variety of oxygenase populations degradin g a broad range of chlorinated solvents are categorized and compared. The d iscussion section of the paper contains an exploration of knowledge gaps th at exist in our understanding of the kinetics of aerobic chlorinated solven t cometabolism. Specific topics covered include: . the use of half saturation constants (K-sc and K-sg) as estimates for inh ibition constants (K-isc and K-isg) in saturation modeling expressions, . the specific nature of chlorinated solvent induced product toxicity and t he capability for cells to recover from toxic effects, and . methods for incorporating reducing energy limitations into cometabolism m odels. Finally, the applicability of the broad range of kinetic modeling approache s to scale-up and field applications for in situ bioremediation of chlorina ted solvents is discussed.