Confocal imaging of HuC/D RNA-binding proteins in adult rat primary sensory neurons

Citation
M. Fornaro et S. Geuna, Confocal imaging of HuC/D RNA-binding proteins in adult rat primary sensory neurons, ANN ANATOMY, 183(5), 2001, pp. 471-473
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
ISSN journal
0940-9602 → ACNP
Volume
183
Issue
5
Year of publication
2001
Pages
471 - 473
Database
ISI
SICI code
0940-9602(200109)183:5<471:CIOHRP>2.0.ZU;2-S
Abstract
HuC/D RNA-binding proteins are antigens that are specifically expressed in nerve cells. In the present study, immunocytochemistry and laser confocal m icroscopy were used to study the cellular distribution of HuC/D RNA-binding proteins in adult rat primary sensory neurons. Colocalization with the pro tein gene product 9.5 (PGP 9.5) was used to identify sensory neurons. Confo cal laser imaging showed that HuC/D antigens are expressed in the cytoplasm of all rat primary sensory neurons. Most neurons also showed anti-HuC/D im muno-positivity in the nucleus. The demonstration of the ubiquitous express ion of HuC/D antigens in primary sensory neurons gives us a possible explan ation for the severe pathological lesions observed in dorsal root ganglia i n association with the paraneoplastic Hu syndrome.