A new panoptic stain for developmental biology - The mouse placenta paradigm

Citation
H. Kurz et D. Wittekind, A new panoptic stain for developmental biology - The mouse placenta paradigm, ANN ANATOMY, 183(5), 2001, pp. 437-441
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
ISSN journal
0940-9602 → ACNP
Volume
183
Issue
5
Year of publication
2001
Pages
437 - 441
Database
ISI
SICI code
0940-9602(200109)183:5<437:ANPSFD>2.0.ZU;2-Z
Abstract
A panoptic histological stain, PHTA, is introduced for routine use in devel opmental biology. The protocol is based on three dyes, hematoxylin and, aft er tannic acid treatment, phloxine B and azure B. It was optimized for diff erential staining of extracellular matrix, cytoplasm and chromatin. The met hod is quick, inexpensive and well-reproducible. The mouse placenta is used here to demonstrate the excellent suitability of PHTA for routine morpholo gy.